| Product name | SDHA Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human SDHA |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-300 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | SDHA; SDH2; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 70kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | SDHA encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Gene ID | 6389 |
| Alternative | SDHA; SDH2; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp |
| Others | SDHA Polyclonal Antibody detects endogenous levels of SDHA protein. |
| Accession | P31040 |
| Observed Band(KD) | 70 |
Fig.1. Western Blot analysis of various cells using SDHA Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of HepG2 cells using SDHA Polyclonal Antibody diluted at 1:2000.
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