| Product name | Rubisco Polyclonal Antibody |
| Immunogen | Purified Protein |
| Host | Mouse |
| Reactivity | VariousPlants |
| Applications | WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:3000-5000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from mouse antiserum by affinity-chromatography using specific immunogen. |
| Alternative | Rubisco; RuBisCO large subunit; Ribulose bisphosphate carboxylase large chain |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. |
| Alternative | Rubisco; RuBisCO large subunit; Ribulose bisphosphate carboxylase large chain |
| Others | The antibody detects endogenous Rubisco Large Chain protein. |
Fig. Western blot analysis of Arabidopsis thaliana, diluted at 1:5000. Secondary antibody was diluted at 1:20000.
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