| Product name | RT14 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human RT14 protein at amino acid sequence of 50-130 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:5000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | 28S ribosomal protein S14, mitochondrial; MRP-S14; S14mt; Mitochondrial small ribosomal subunit protein uS14m |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 14kD |
| Storage buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS14 encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. |
| Gene ID | 63931 |
| Alternative | 28S ribosomal protein S14, mitochondrial; MRP-S14; S14mt; Mitochondrial small ribosomal subunit protein uS14m |
| Others | The antibody detects endogenous levels of RT14 protein |
| Accession | O60783 |
| Observed Band(KD) | 14 |
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