| Product name | RCC1 Polyclonal Antibody |
| Immunogen | Synthetic peptide from human protein at AA range: 301-350 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-200;ELISA 1:10000-20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | RCC1; CHC1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 45kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport. Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. RCC1 (regulator of chromatin condensation 1) is the only known RanGEF. RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally. Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation and methylation. RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis. In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclinB/cdc2. This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity. |
| Gene ID | 1104 |
| Alternative | RCC1; CHC1 |
| Others | The antibody detects endogenous RCC1 protein. |
| Accession | P18754 |
| Observed Band(KD) | 45 |
Fig.1. Western Blot analysis of HEPG2 cells using Antibody diluted at 1:800. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-thyroid, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.
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