Product name | Rat SCF Protein, His tag |
Sequence | Amino acid sequence derived from Recombinant Rat SCF Protein P21581-1 (Q26-A189) was expressed. |
Activity | Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 0.1737 ng/mL. |
Protein length | The Recombinant Rat SCF Protein consists of 170 amino acids and predicts a molecular mass of 18.47 KD. |
Preparation method | E. coli |
Purity | > 99 % as determined by SDS-PAGE |
Alternative | Stem Cell Factor;SCF;KITLG;MGF;SF;KL1;Kitl;FPH2;Kit-Ligand;Steel Factor;Mast Cell Growth Factor;Steel factor;Familial Progressive Hyperpigmentation 2 |
Formulation | Lyophilized from sterile 20mM Tris,0.15M NaCl, pH 7.4. |
Molecular weight | 18.47 kDa |
Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-3 protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage instructions | Lyophilized Rat SCF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | Stem cell factor (SCF), as a key ligand of KIT, regulates cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis. Binding to KIT activates signaling pathways involving phosphorylation events, key kinases (AKT1, RAS, RAF1, MAPK1/ERK2, MAPK3/ERK1), STAT family members, and PLCG1. SCF Protein, Rat (His) is the recombinant rat-derived SCF protein, expressed by E. coli with C-6*His labeled tag. |
Alternative | Stem Cell Factor;SCF;KITLG;MGF;SF;KL1;Kitl;FPH2;Kit-Ligand;Steel Factor;Mast Cell Growth Factor;Steel factor;Familial Progressive Hyperpigmentation 2 |
Accession | P21581-1 |
Fig.Rat SCF Protein, His tag
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