| Product name | Rat G-CSF Protein, His tag |
| Sequence | Amino acid sequence derived from Rat G-CSF(P97712)(Ile22-Ile214) was expressed with 6×His tag at N-terminus. |
| Applications | His tag |
| Applications notes | Rat |
| Activity | Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 4.802 ng/mL. |
| Protein length | The Rat G-CSF consists of 193 amino acids and predicts a molecular mass of 21.3 kDa. |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE |
| Alternative | G-CSF;CSF-3; MGI-1G; GM-CSF beta; MGC45931; Pluripoietin |
| Formulation | Liquid in from sterile 20mM Tris, 250mM NaCl, 10% Glycerol, pH 8.0 |
| Features & Benefits | Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 21.3 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to diluted the protein using the PBS buffer or Protein storage solution to working concentration |
| Storage instructions | The Rat G-CSF protein product should be stored desiccated below -20°C. The protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | G-CSF is a hematopoietic growth factor, which controls the production, differentiation and function of granulocytes and enhances the functional activity of mature terminal cells. Three transcription variants of G-CSF gene encoding three different subtypes have been found. Granulocyte/macrophage colony stimulating factor is a cytokine that plays a role in hematopoiesis by controlling the production, differentiation and function of two related leukocyte populations (granulocyte and monocyte-macrophage) in blood. G-CSF is clinically used to promote hematopoietic recovery after bone marrow transplantation. |
| Gene ID | 25610 |
| Alternative | G-CSF;CSF-3; MGI-1G; GM-CSF beta; MGC45931; Pluripoietin |
| Accession | P97712 |
Fig.Rat G-CSF Protein.
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