| Product name | Rat CNTF Protein |
| Sequence | Amino acid sequence derived from RatCNTF（P20294）（Met 1-Met 200） was expressed . |
| Activity | Testing in progress. |
| Protein length | The recombinant Rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa.It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions. |
| Preparation method | E. coli |
| Purity | > 97 % as determined by SDS-PAGE |
| Alternative | Ciliary neurotrophic factor;CNTF;H-CNTF |
| Formulation | Lyophilized from sterile 20 mM Tris 50mM NaCl ,pH 8.0 |
| Features & Benefits | <1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 22.9 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat-CNTF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Rat-CNTF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Gene ID | 25707 |
| Alternative | Ciliary neurotrophic factor;CNTF;H-CNTF |
| Accession | P20294 |
Fig.SDS-PAGE analysis of Rat CNTF Protein
Yes,It can be crushed by ultrasonic.The reference ultrasonic conditions are as follows: ice bath ultrasonication for 5 min (power 20%, ultrasonication for 3 s, interval of 7 s, repeated 30 times).
Store the supernatant and pellet together at -80°C, pay attention to aliquoting and avoid repeated freezing and thawing. It is recommended to use fresh samples for experiments whenever possible.
Reduced cytochrome C has characteristic light absorption at 550nm. Mitochondrial respiratory chain complex IV could catalyze reduced cytochrome C to oxidized cytochrome C. Therefore, the decreasing rate of light absorption at 550nm can reflect the enzyme activity of mitochondrial respiratory chain complex IV.
Yes,It can be crushed by ultrasonic.The reference ultrasonic conditions are as follows: ice bath ultrasonication for 5 min (power 20%, ultrasonication for 3 s, interval of 7 s, repeated 30 times).
Store the supernatant and pellet together at -80°C, pay attention to aliquoting and avoid repeated freezing and thawing. It is recommended to use fresh samples for experiments whenever possible.
Complex Ⅴ hydrolyzes ATP to produce ADP and Pi. The activity of complex Ⅴ is determined by measuring Pi increase rate.
Yes,It can be crushed by ultrasonic.The reference ultrasonic conditions are as follows: ice bath ultrasonication for 5 min (power 20%, ultrasonication for 3 s, interval of 7 s, repeated 30 times).
Yes, you need to use a 96-well UV plate,because the wavelength to be measured at 340 nm is not visible light, but is ultraviolet, so you need to use a UV plate for measurement.
Store the supernatant and pellet together at -80°C, pay attention to aliquoting and avoid repeated freezing and thawing. It is recommended to use fresh samples for experiments whenever possible.
Mitochondrial respiratory chain complex I can catalyze the dehydrogenation of NADH to NAD+, and directly read the oxidation rate of NADH under 340nm to calculate the activity of the enzyme.
Author： Publication name： IF：
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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