| Product name | Rad51 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Rad51 around the non-phosphorylation site of Y315 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | RAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 42kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | RAD51 encoded by RAD51（RAD51 recombinase) is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for RAD51. |
| Gene ID | 5888 |
| Alternative | RAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A |
| Others | Rad51 Polyclonal Antibody detects endogenous levels of Rad51 protein. |
| Accession | Q06609 |
Fig.1. Western Blot analysis of various cells using Rad51 Polyclonal Antibody diluted at 1:500.
Fig.2. Western Blot analysis of hela-UV cells using Rad51 Polyclonal Antibody diluted at 1:500.
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