| Product name | PU.1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human PU.1 at AA range: 150-230 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | SPI1; Transcription factor PU.1; 31 kDa-transforming protein |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 32kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | SPI1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. Spi-1 proto-oncogene can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this SPI1. |
| Gene ID | 6688 |
| Alternative | SPI1; Transcription factor PU.1; 31 kDa-transforming protein |
| Others | PU.1 Polyclonal Antibody detects endogenous levels of PU.1 protein. |
| Accession | P17947 |
| Observed Band(KD) | 32 |
Fig. Western Blot analysis of various cells using PU.1 Polyclonal Antibody.
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