| Product name | PP2A-Cα (phospho Tyr307) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human PP2A-Cα around the phosphorylation site of Y307 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IP,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 35kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | PPP2CA (protein phosphatase 2 catalytic subunit alpha) encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. PPP2CA encodes an alpha isoform of the catalytic subunit. |
| Gene ID | 5515 |
| Alternative | PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C |
| Others | Phospho-PP2A-Cα (Y307) Polyclonal Antibody detects endogenous levels of PP2A-Cα protein only when phosphorylated at Y307. |
| Accession | P67775 |
| Observed Band(KD) | 35 |
Fig.1. Western blot analysis of SKOV3 using p-PP2A-Cα (Y307) antibody. Antibody was diluted at 1:1000.
Fig.2. Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.
Author：Guoqing Hou Publication name：Evid Based Complement Alternat Med IF：5.6
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