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POLH Polyclonal Antibody

POLH Polyclonal Antibody

Views(3) Publications(0) Catalog no(ABP59967)
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Specification

Product name POLH Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human POLH protein
Host Rabbit
Reactivity Human,Mouse
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:5000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative DNA polymerase eta; RAD30 homolog A; Xeroderma pigmentosum variant type protein

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 78kD
Storage buffer PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background POLH encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.
Gene ID 5429
Alternative DNA polymerase eta; RAD30 homolog A; Xeroderma pigmentosum variant type protein
Others The antibody detects endogenous levels of POLH protein
Accession Q9Y253
Observed Band(KD) 78

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