| Product name | PKD2 (phospho Ser876) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human PKD2 around the phosphorylation site of S876 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | PRKD2; PKD2; HSPC187; Serine/threonine-protein kinase D2; nPKC-D2 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 96kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Protein kinase D2 encoded by PRKD2 belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. Protein kinase D2 can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Gene ID | 25865 |
| Alternative | PRKD2; PKD2; HSPC187; Serine/threonine-protein kinase D2; nPKC-D2 |
| Others | Phospho-PKD2 (S876) Polyclonal Antibody detects endogenous levels of PKD2 protein only when phosphorylated at S876. |
| Accession | Q9BZL6 |
| Observed Band(KD) | 96 |
Fig.1. Western Blot analysis of various cells using Phospho-PKD2 (S876) Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of HELA cells using Phospho-PKD2 (S876) Polyclonal Antibody diluted at 1:1000.
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