| Product name | PI 3-kinase p85α Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human PI 3-kinase p85α protein at amino acid sequence of 550-630 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat,Pig |
| Applications | IF,WB,IHC,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;Not yet tested in other applications; |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 54kD,83kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | PIK3R1 (Phosphoinositide-3-Kinase Regulatory Subunit 1) is a Protein Coding gene. Diseases associated with PIK3R1 includeShort SyndromeandAgammaglobulinemia 7, Autosomal Recessive. Among its related pathways are4-1BB PathwayandE-cadherin signaling in keratinocytes. |
| Gene ID | 5295 |
| Alternative | PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph |
| Others | This antibody detects endogenous levels of PI 3-kinase p85α. |
| Accession | P27986 |
| Observed Band(KD) | 54+83 |
Fig.1. Western Blot analysis of various cells using primary antibody diluted at 1:500 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.
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