| Product name | PFK-2 car (phospho Ser483) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human PFK-2 car around the phosphorylation site of S483 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | PFKFB2; 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 58kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 encoded by PFKFB2 is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for PFKFB2. |
| Gene ID | 5208 |
| Alternative | PFKFB2; 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme |
| Others | Phospho-PFK-2 car (S483) Polyclonal Antibody detects endogenous levels of PFK-2 car protein only when phosphorylated at S483. |
| Accession | O60825 |
| Observed Band(KD) | 58 |
Fig.1. Western Blot analysis of various cells using Phospho-PFK-2 car (S483) Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of Hela cells using Phospho-PFK-2 car (S483) Polyclonal Antibody diluted at 1:1000.
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