| Product name | Perforin 1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from Perforin 1 at AA range: 451-500 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-200;ELISA 1:10000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; PFP |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 61kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The protein encoded by PRF1 (perforin 1) has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in PRF1 cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. |
| Gene ID | 5551 |
| Alternative | Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; PFP |
| Others | Perforin 1 Polyclonal Antibody detects endogenous levels of Perforin 1. |
| Accession | P14222 |
| Observed Band(KD) | 61 |
Fig.1. Western Blot analysis of Hela cells using Perforin 1 Polyclonal Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.
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