| Product name | PDK3 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human PDK3 protein at amino acid sequence of 170-250 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:5000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial ; Pyruvate dehydrogenase kinase isoform 3 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 44kD |
| Storage buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by PDK3 (Pyruvate Dehydrogenase Kinase 3) is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. PDK3 is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for PDK3. |
| Gene ID | 5165 |
| Alternative | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial ; Pyruvate dehydrogenase kinase isoform 3 |
| Others | The antibody detects endogenous levels of PDK3 protein |
| Accession | Q15120 |
| Observed Band(KD) | 44 |
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