| Product name | PBFE Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human PBFE at AA range: 450-530 |
| Host | Rabbit |
| Reactivity | Human,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | EHHADH; ECHD; Peroxisomal bifunctional enzyme; PBE; PBFE |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 80kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase by EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in EHHADH are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for EHHADH. |
| Gene ID | 1962 |
| Alternative | EHHADH; ECHD; Peroxisomal bifunctional enzyme; PBE; PBFE |
| Others | PBFE Polyclonal Antibody detects endogenous levels of PBFE protein. |
| Accession | Q08426 |
| Observed Band(KD) | 80 |
Fig.1. Western Blot analysis of various cells using PBFE Polyclonal Antibody.
Fig.2. Western Blot analysis of A549 cells using PBFE Polyclonal Antibody.
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