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Parafibromin Polyclonal Antibody

Parafibromin Polyclonal Antibody

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Specification

Product name Parafibromin Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human Parafibromin protein at amino acid sequence of 51-100
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Parafibromin; Cell division cycle protein 73 homolog; Hyperparathyroidism 2 protein

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 60kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CDC73 encodes a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. The protein is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. This protein appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin. Mutations in CDC73 have been linked to hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism, and parathyroid carcinoma.
Gene ID 79577
Alternative Parafibromin; Cell division cycle protein 73 homolog; Hyperparathyroidism 2 protein
Others This antibody detects endogenous levels of Parafibromin
Accession Q6P1J9
Observed Band(KD) 60

Image & description

Fig.1. Western Blot analysis of 1, Mouse-liver 2, Mouse-brain cells using primary antibody diluted at 1:1000 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.

Fig.1. Western Blot analysis of 1, Mouse-liver 2, Mouse-brain cells using primary antibody diluted at 1:1000 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.

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