| Product name | p300 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human p300 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | IF,WB,IHC,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200;WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:10000;Not yet tested in other applications; |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 300kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | EP300 (E1A binding protein p300) encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in EP300 are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. |
| Gene ID | 2033 |
| Alternative | EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300 |
| Others | p300 Polyclonal Antibody detects endogenous levels of p300 protein. |
| Accession | Q09472 |
| Observed Band(KD) | 300 |
Fig.1. Immunofluorescence analysis of rat kidney tissue. 1, p300 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.2. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, p300 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, p300 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, p300 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.5. Western blot analysis of Hela (1), 293T (2), 823-AV (3), diluted at 1:1000.
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