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ODPB Polyclonal Antibody

ODPB Polyclonal Antibody

Views(3) Publications(0) Catalog no(ABP59639)
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Specification

Product name ODPB Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human ODPB protein at amino acid sequence of 160-240
Host Rabbit
Reactivity Human,Rat,Mouse
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:5000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; PDHE1-

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 39kD
Storage buffer PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. PDHB (Pyruvate Dehydrogenase E1 Beta Subunit) encodes the E1 beta subunit. Mutations in PDHB are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for PDHB.
Gene ID 5162
Alternative Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; PDHE1-
Others The antibody detects endogenous levels of ODPB protein
Accession P11177
Observed Band(KD) 39

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