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ND1 Polyclonal Antibody

ND1 Polyclonal Antibody

Views(3) Publications(0) Catalog no(ABP51905)
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Specification

Product name ND1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human ND1 at AA range: 150-230
Host Rabbit
Reactivity Human,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative MT-ND1; MTND1; NADH1; ND1; NADH-ubiquinone oxidoreductase chain 1; NADH dehydrogenase subunit 1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 36kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MT-ND1 is a Protein Coding gene. Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Diseases associated with MT-ND1 include melas syndrome and leber hereditary optic neuropathy. Among its related pathways are Metabolism and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins. Gene Ontology (GO) annotations related to this gene include NADH dehydrogenase (ubiquinone) activity.
Gene ID 4535
Alternative MT-ND1; MTND1; NADH1; ND1; NADH-ubiquinone oxidoreductase chain 1; NADH dehydrogenase subunit 1
Others ND1 Polyclonal Antibody detects endogenous levels of ND1 protein.
Accession P03886
Observed Band(KD) 36

Image & description

Fig.1. Western Blot analysis of various cells using ND1 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using ND1 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of COLO205 cells using ND1 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of COLO205 cells using ND1 Polyclonal Antibody diluted at 1:1000.

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