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NCAM-L1 Polyclonal Antibody

NCAM-L1 Polyclonal Antibody

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Specification

Product name NCAM-L1 Polyclonal Antibody
Immunogen Synthesized peptide derived from human NCAM-L1 around the non-phosphorylation site of S1181
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative L1CAM; CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD antigen CD171

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 180kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Neural cell adhesion molecule L1 encoded by L1CAM is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.
Gene ID 3897
Alternative L1CAM; CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD antigen CD171
Others NCAM-L1 Polyclonal Antibody detects endogenous levels of NCAM-L1 protein.
Accession P32004
Observed Band(KD) 180

Image & description

Fig.1. Western Blot analysis of various cells using NCAM-L1 Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using NCAM-L1 Polyclonal Antibody.

Fig.2. Western Blot analysis of Jurkat cells using NCAM-L1 Polyclonal Antibody.

Fig.2. Western Blot analysis of Jurkat cells using NCAM-L1 Polyclonal Antibody.

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