| Product name | Mycoplasma PCR Detection Kit |
| Applications | Mycoplasma detection |
| SampleType | Cell culture supernatant, laboratory animal secretions, serum and other biomaterial samp |
| Assay type | PCR |
| Assay duration | 4-5 h |
| Formulation | Liquid solution |
| Kit components | •1st PCR Primer Mix (25×) •2nd PCR Primer Mix (25×) •Positive Control Template |
| Features & Benefits | • Compared with luminescence method and DNA staining method, PCR method has higher sensitivity, strong specificity and easy operation. • Mycoplasma species can be accurately identified according to the size of the 1st and 2nd PCR products. |
| Usage notes | • In order to prevent contamination of the Positive Control Template, it is necessary to add the Positive Control Template to the positive control group after adding samples to the test group and negative control group. |
| Storage instructions | Stored at -20℃ for 12 months. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mycoplasma is the smallest and simplest prokaryote. It has the following characteristics: there is no cell wall structure, and many common antibiotics that target the cell wall, such as penicillin or beta-lactam antibiotics, are ineffective against mycoplasma; The size is between bacteria and viruses, about 0.2-0.8μm, some mycoplasma can pass 0.22 μm filter, conventional filtration is ineffective for mycoplasma; Due to their limited biosynthesis capacity, many mycoplasma are usually adsorbated or scattered on the cell surface and between cells, relying on the host for nutrition. Mycoplasma pollution may seriously affect the state of cells, causing changes in gene expression and metabolic characteristics of cells, resulting in abnormal cell growth, differentiation and death, and seriously affecting cell function. At the same time, the reliability, repeatability and consistency of the experimental results are seriously affected, so the detection of mycoplasma contamination is very important. |
Fig1. Mycoplasma PCR Detection Kit
Fig2. Agarose gel electrophoresis diagram for PCR detection of amplified products. 1, 2 and 3 were the products of 1st PCR. 1#, 2#, 3# were the corresponding 2nd PCR products. The templates for each lane are: 1 and 1#, Positive Control Template; 2 and 2#, mycoplasma contaminated cell supernatant; 3 and 3# Sterilized water. M stands for DNA marker.
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