| Product name | MRP-S34 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human MRP-S34 at AA range: 160-240 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA,IHC |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | MRPS34; 28S ribosomal protein S34; mitochondrial; MRP-S34; S34mt |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 26kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | 28S ribosomal protein S34 mitochondrial (mitochondrial ribosomal protein S34) is encoded by nuclear genes MRPS34 and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternative splicing results in multiple transcript variants. |
| Gene ID | 65993 |
| Alternative | MRPS34; 28S ribosomal protein S34; mitochondrial; MRP-S34; S34mt |
| Others | MRP-S34 Polyclonal Antibody detects endogenous levels of MRP-S34 protein. |
| Accession | P82930 |
| Observed Band(KD) | 26 |
Fig.1. Western Blot analysis of various cells using MRP-S34 Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of Jurkat cells using MRP-S34 Polyclonal Antibody diluted at 1:1000.
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