| Product name | Mouse VEGF165 Protein |
| Sequence | Amino acid sequence derived from Mouse VEGF165 (Q00731)(Ala228-Arg392) was expressed |
| Applications notes | Mouse |
| Activity | Measured in a cell proliferation assay using HUVEC-SV40. The ED50 for this effect is typically 0.30 ng/mL. The specific activity of recombinant Mouse VEGF165 is approximately >3.33 x 10 6 IU/mg. |
| Protein length | The recombinant Mouse VEGF165 consists of 165 amino acids and predicts a molecular mass of 19.3 kDa. |
| Preparation method | E. coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Alternative | MVCD1; VEGF; VEGF165; VPF; Vascular endothelial growth factor A; VEGFA |
| Formulation | Lyophilized from sterile 20 mM Tris-HCl, 150mM NaCl, pH7.5. |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 19.3 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to diluted the protein using the PBS buffer or Protein storage solution to working concentration |
| Storage instructions | The recombinant Mouse VEGF165 protein product should be stored desiccated below -20°C. The protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Vascular Endothelial Growth Factors 165 (VEGF165) is a potent growth and angiogenic cytokine which belongs to the VEGF family, includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF165 is an abundant glycosylated cytokine composed of two identical 165 amino acid chains.VEGF165 plays an important role in embryonic vasculogenesis, angiogenesis and neurogenesis |
| Gene ID | 22339 |
| Alternative | MVCD1; VEGF; VEGF165; VPF; Vascular endothelial growth factor A; VEGFA |
| Accession | Q00731 |
Fig.SDS-PAGE analysis of Mouse VEGF165 Protein
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