| Product name | Mouse VEGF121 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse VEGF121 (Ala 205-Arg 392) (Q00731) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measured by its ability to induce proliferation in HUVEC cells. The ED50 for this effect is <3 ng/mL |
| Protein length | The recombinant Mouse VEGF121 consists of 188 amino acids and predicts a molecular mass of 15.01 kDa |
| Preparation method | E. coli |
| Purity | >95% as determined by SDS-PAGE. |
| Alternative | VPF |
| Formulation | Lyophilized from PBS, pH 8.0 |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 15.01 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Vascular Endothelial Growth Factors 121 (VEGF121) is a truncated version of VEGF165, which produced in E. coli is a homodimer, non-glycosylated, polypeptide chain and having a molecular mass of 28.4 kDa. There is three different isoforms (120, 164 and 188 a.a.) found in mouse. VEGF 121 shows that lack basic heparin-binding regions and are freely diffusible. Mouse VEGF121 shares 98% identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively. |
| Alternative | VPF |
| Accession | Q00731(Mouse), P16612(Rat) |
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