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Mouse/Rat VEGF165 Protein, His tag (Animal-Free)

Mouse/Rat VEGF165 Protein, His tag (Animal-Free)

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Specification

Product name Mouse/Rat VEGF165 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Mouse/Rat VEGF 165 Protein (Met 3530Arg 3668)(NP_0335313) was expressed was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Mouse
Activity Measure by its ability to induce proliferation in HUVEC cells.The ED₅₀ for this effect is <3 ng/mL.
Protein length The protein has a calculated MW of 20.22 kDa.The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Preparation method E. coli
Purity >98% as determined by SDS-PAGE.
Alternative VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen

Product Properties

Formulation The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight The protein has a calculated MW of 20.22 kDa.The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse/Rat VEGF 165 Protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Beta-Nerve Growth Factors (Beta-NGF) is a 27 kDa cytokine with 241 amino acid residues. Beta-NGF belongs to neurotrophin family, and acts as neurotrophic factors. It's composed of alpha, beta, gamma subnuits, and the beta subunit is related to its biological activity. Beta-NGF binds to p75 neurotrophin receptor and Trk receptor and their function is about cell death and survival, respectively
Alternative VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen
Accession NP_033531 3

Image & description

Fig.SDS-PAGE analysis of Mouse/Rat VEGF165 protein

Fig.SDS-PAGE analysis of Mouse/Rat VEGF165 protein

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