| Product name | Mouse M-CSF protein,His tag |
| Sequence | Amino acid sequence derived from Mouse M-CSF (Lys33-Glu262 ) (P07141-1) was expressed with 6×His tag. |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 0.1179 ng/mL. |
| Protein length | The recombinant Mouse M-CSF consists of 230 amino acids and predicts a molecular mass of 26.1 kDa. |
| Preparation method | E.coli |
| Purity | > 95% as determined by SDS-PAGE |
| Molecular Formula | 26 kDa |
| Alternative | Macrophage colony-stimulating factor 1; CSF-1; MCSF; Csf1; Csfm |
| Formulation | Lyophilized from 20 mM Tris-HCl, 150mM NaCl, pH8.5. |
| Features & Benefits | Endotoxin :< 0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 26 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions |
| Storage instructions | Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis |
| Gene ID | 12977 |
| Alternative | Macrophage colony-stimulating factor 1; CSF-1; MCSF; Csf1; Csfm |
| Accession | P07141-1 |
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