| Product name | Mouse Leptin Protein |
| Sequence | Recombinant Mouse Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed. |
| Activity | Immobilized Recombinant Mouse LEPR (C-10His) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin is 1.16 ng/ml.(Regularly tested). |
| Protein length | The recombinant Leptin Protein consists of 146 amino acids with a Met in N terminal and predicts a molecular mass of 16 kDa. |
| Preparation method | E. coli |
| Purity | >95% as determined by SDS-PAGE |
| Alternative | Leptin; Obese Protein; Obesity Factor; LEP; OB; OBS |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH7.5. |
| Features & Benefits | Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 16 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Leptin protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized recombinant Leptin protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. Animal models have revealed the influence of Leptin in reducing body weight and regulating blood glucose level. When mutations are introduced in obese gene, mice with impaired function of leptin are massively obese and in high risk of diabetes. Leptin deficiency reduces metablic rate. Leptin deficient mice are less active and with lower body temperature than normal animals. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin consists of 167 amino acid residue including a 21 amino acid residue signal sequence and 146 amino acid residue mature protein sequence. |
| Gene ID | 16846 |
| Alternative | Leptin; Obese Protein; Obesity Factor; LEP; OB; OBS |
Fig.SDS-PAGE analysis of Mouse Leptin Protein
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