| Product name | Mouse IL-6 protein |
| Sequence | Amino acid sequence derived from Mouse IL-6 (Phe25-Thr211) (P08505) was expressed. |
| Applications | No tag |
| Applications notes | Mouse |
| Activity | Measured in a cell proliferation assay using M-NFS60. The ED50 for this effect is typically 1.75 ng/mL. The specific activity of recombinant Mouse IL6 is approximately >5.71 x 105IU/mg. |
| Protein length | The recombinant Mouse IL-6 consists of 187 amino acids and predicts a molecular mass of 21.9 kDa |
| Preparation method | E.coli |
| Purity | > 99 % as determined by SDS-PAGE |
| Molecular Formula | 21.9 kDa |
| Formulation | Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.4. |
| Molecular weight | 21.9 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. Mouse IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 in regulating the acute phase response. Mouse IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 has a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants |
| Gene ID | 16193 |
| Accession | P08505 |
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