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Mouse IL-25 Protein, His tag (Animal-Free)

Mouse IL-25 Protein, His tag (Animal-Free)

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Specification

Product name Mouse IL-25 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Mouse IL-25 (Val 17-Ala 169) (NP_542767.1) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Mouse
Activity Measured by its ability to induce CXCL1 secretion in HT‑ 29 cells.The ED50 for this effect is <1 ng/mL
Protein length The recombinant Mouse IL-25 consists of 152 amino acids and predicts a molecular mass of 18.41 kDa
Preparation method E. coli
Purity >98% as determined by SDS-PAGE.
Alternative IL-17E, Il17e

Product Properties

Formulation Lyophilized from 20 mM sodium citrate, 0.2 M NaCl, pH 4.5
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 18.41 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 25 (IL-25) is a cytokine with proinflammatory and anti-inflammatory effects, it predicts a molecular mass of 16.7 kDa. It can induce NF-κB activation, and stimulate the production of IL-8, which is the major chemotactic substance of neutrophils.
Alternative IL-17E, Il17e
Accession NP_542767 1

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