| Product name | Mouse IL-21 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse IL-21 (His18-Ser146) (Q9ES17) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measure by its ability to enhance IFN gamma secretion in NK-92 cells.The ED₅₀ for this effect is <6 ng/mL.The specific activity of recombinant mouse IL-21 is > 1.6 x 10⁵ IU/mg. |
| Protein length | The recombinant Mouse IL-21 Protein consists of 129 amino acids and predicts a molecular mass of 15.9 kDa |
| Preparation method | E. coli |
| Purity | > 95% as determined by SDS-PAGE. |
| Alternative | Za11 |
| Formulation | The protein was lyophilized from sterile PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 15.9 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-21 (IL-21) belongs to the IL-15/IL-21 family, which exerts pleiotropic immune regulations. IL-21 produced primarily by natural killer T (NKT) cells, T follicular helper (TFH) cells and TH17 cells. As a pleiotropic cytokine, IL-21 has been shown to regulate both innate and humoral immunity. It has potent inhibitory activity towards the activation and maturation of granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced dendritic cells (DCs). In B cells, IL-21 has a major role in the development of immunoglobulin responses. In T cells, it is required to facilitate the functional differentiation of several CD4+ T cell subsets. In addition, the ability of IL-21 to enhance the cytotoxic activity of both CD8+ T cells and NK cells makes it as a potential antitumor agent. |
| Alternative | Za11 |
| Accession | Q9ES17 |
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