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Mouse IL-18 Protein, His tag (Animal-Free)

Mouse IL-18 Protein, His tag (Animal-Free)

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Specification

Product name Mouse IL-18 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Mouse IL-18 (Met 36-Asn 192) (P70380) was expressed with 6×His tag and a Met at the C-terminus
Applications His tag
Applications notes Mouse
Activity Measure by its ability to induce IFN gamma secretion in KG-1 cells.The ED₅₀ for this effect is <0.5 μg/mL.
Protein length The protein has a calculated MW of 19.1 kDa.The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Preparation method E. coli
Purity > 98% as determined by SDS-PAGE.
Alternative IGIF

Product Properties

Formulation The protein was lyophilized from sterile PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 19.1 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping The product is shipped with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 18(IL-18), also known as interferon gamma inducible factor, is a pro-inflammatory cytokine that in humans is encoded by the IL-18 gene.IL-18 belong to the IL-1 superfamily and are secreted by macrophages and other cells.Upon IL-18 stimulation,natural killer (NK) cells and some T cells release another important cytokine,interferon-gamma or type ll interferon,this in turn activates macrophages or other cells.IL-18 induces a severe inflammatory response, as well as increased production of amyloid (which is associated with Alzheimer's disease)in human neurons
Alternative IGIF
Accession P70380

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