| Product name | Mouse IL-10 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse IL-10（P18893）（Ser19-Ser178）was expressed with 6×His tag at the C-terminus. |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Testing in progress |
| Protein length | The recombinant Mouse IL-10 consists of 161 amino acids and predicts a molecular mass of 18.9 kDa. |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | B-TCGF, CSIF, TGIF |
| Formulation | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 19.7 kDa. The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis) |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse IL-10 protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway |
| Alternative | B-TCGF, CSIF, TGIF |
| Accession | P18893 |
Fig.SDS-PAGE analysis of Mouse IL-10 protein
You must be logged in to post a review.
Reviews
There are no reviews yet.