| Product name | Mouse IFN-γ protein |
| Sequence | Amino acid sequence derived from Mouse IFN-γ protein soluble form (NP_P01580) (His23-Cys155) was expressed, was expressed with a polyhistidine tag at the N-terminus. |
| Applications | No tag |
| Applications notes | Mouse |
| Activity | Measured in a cell proliferation assay using L929. The ED50 for this effect is typically 0.03 ng/mL. The specific activity of recombinant Mouse IFN-γis approximately >3.33 x 107IU/mg. |
| Protein length | The recombinant Mouse IFN-γ protein consists of 154 amino acids and predicts a molecular mass of 16.9 kDa. |
| Preparation method | E.coli |
| Purity | > 95 % as determined by SDS-PAGE |
| Molecular Formula | 16.9 kDa |
| Formulation | Lyophilized from sterile 150 mM NaCl 20 mM Tris , pH 8.0. |
| Molecular weight | 16.9 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | IFN gamma (IFN-γ), also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN-γ has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity. |
| Gene ID | 15978 |
| Accession | P01580 |
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