| Product name | Mouse GM-CSF Protein |
| Sequence | Amino acid sequence derived from Mouse GM-CSF (Ala18-Lys141)(P01587) was expressed |
| Applications | No tag |
| Applications notes | Mouse |
| Activity | Measured in a cell proliferation assay using FDC-P1 cells.The ED50 for this effect is 1.203 ng/mL. |
| Protein length | The recombinant Mouse GM-CSF consists of 124 amino acids and predicts a molecular mass of 14.2 kDa |
| Preparation method | E. coli |
| Purity | > 96 % as determined by SDS-PAGE |
| Alternative | CSF; CSF2; CSF-2; GMCSF; GM-CSF;colony stimulating factor 2 (granulocyte-macrophage); granulocyte-macrophage colony-stimulating factor; Colony-stimulating factor; GMCSF, granulocyte-macrophage colony stimulating factor; molgramostin; Sargramostim, MGC131935; MGC138897; Molgramostim; |
| Formulation | The protein was liquid in from sterile 20 mM Tris, 150 mM NaCl ,5% Glycerol, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin: <1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 14.2 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to diluted the protein using the PBS buffer or Protein storage solution to working concentration |
| Storage instructions | The recombinant Mouse GM-CSF protein product should be stored desiccated below -20°C.The protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli.Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines |
| Gene ID | 12981 |
| Alternative | CSF; CSF2; CSF-2; GMCSF; GM-CSF;colony stimulating factor 2 (granulocyte-macrophage); granulocyte-macrophage colony-stimulating factor; Colony-stimulating factor; GMCSF, granulocyte-macrophage colony stimulating factor; molgramostin; Sargramostim, MGC131935; MGC138897; Molgramostim; |
| Accession | P01587 |
Fig.SDS-PAGE analysis of Mouse GM-CSF Protein
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