| Product name |
Mouse GM-CSF protein , His tag (Animal-Free) |
| Sequence |
Amino acid sequence derived from Mouse GM-CSF (Ala18-Lys141)(P01587) was expressed with 6×His tag at the N-terminus. |
| Applications |
His tag |
| Applications notes |
Mouse |
| Activity |
Measure by its ability to induce proliferation in FDC-P1 cells.The ED₅₀ for this effect is <50 pg/mL.The specific activity of recombinant mouse GM-CSF is approximately >2 x 10⁷ IU/mg. |
| Protein length |
The recombinant Mouse GM-CSF consists of 124 amino acids and predicts a molecular mass of 15.1 KDa |
| Preparation method |
E. coli |
| Purity |
>98% as determined by SDS-PAGE. |
| Alternative |
colony stimulating factor 2, CSF2, CSF, Csfg, Csfgm, GMCS, GMCSF, Gm-CS, Gm-CSf, MGI-I, MGI-IGM |
| Formulation |
The protein was Lyophilized from sterile PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits |
Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight |
The protein has a calculated MW of 15.1 kDa.
The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes |
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse GM-CSF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions |
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping |
Gel pack with blue ice. |
| Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background |
Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a Growth Factors due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine. |
| Alternative |
colony stimulating factor 2, CSF2, CSF, Csfg, Csfgm, GMCS, GMCSF, Gm-CS, Gm-CSf, MGI-I, MGI-IGM |
| Accession |
P01587 |
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