| Product name | Mouse G-CSF protein,His tag |
| Sequence | Amino acid sequence derived from Mouse G-CSF (Val31-Ala208) (P09920) was expressed with 6×His tag. |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 1.916 ng/mL. |
| Protein length | The recombinant Mouse G-CSF consists of 178 amino acids and predicts a molecular mass of 19.8 kDa. |
| Preparation method | E.coli |
| Purity | > 98% as determined by SDS-PAGE |
| Molecular Formula | 19.8 kDa |
| Alternative | Granulocyte colony-stimulating factor; Csf3; G-CSF |
| Formulation | Lyophilized from sterile 20mM Tris, 150mM NaCl, 5% Sucrose, 5% Mannitol, pH9.0. |
| Features & Benefits | Endotoxin :< 0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 19.8 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse G-CSF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions |
| Storage instructions | Lyophilized recombinant Mouse G-CSF protein product should be stored desiccated below -20°C.Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Granulocyte colony-stimulating factor (G-CSF) is a growth factor and an essential cytokine which belongs to the IL-6 superfamily. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent |
| Gene ID | 12985 |
| Alternative | Granulocyte colony-stimulating factor; Csf3; G-CSF |
| Accession | P09920 |
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