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Mouse Flt-3 Ligand protein , His tag (Animal-Free)

Mouse Flt-3 Ligand protein , His tag (Animal-Free)

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Specification

Product name Mouse Flt-3 Ligand protein , His tag (Animal-Free)
Sequence Amino acid sequence derived from Mouse Flt-3 Ligand (Gly27-Arg188)(P49772) with 6×His tag at the C-terminus was expressed
Applications His tag
Applications notes Mouse
Activity Measure by its ability to induce proliferation in BaF3 cells transfected with Mouse Flt-3 Ligand.The ED50 for this effect is <2 ng/mL
Protein length The secreted Mouse Flt-3 Ligand consists of 162 amino acids and has a predicted molecular mass of 19.3 kDa
Preparation method E. coli
Purity >98% as determined by SDS-PAGE.
Alternative Fetal liver kinase 2, FLK-2

Product Properties

Formulation The protein was Lyophilized from sterile PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight The protein has a calculated MW of 19.3 kDa.The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse Flt-3 Ligand protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve.Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Fms-associated tyrosine kinase 3 ligand (Flt-3L) is a one-way type I membrane protein. Flt3-L is a hematopoietic quadruplex bundle cytokine, which is structurally homologous to stem cell factor and colony stimulating factor. Flt-3L has a good synergistic effect with many other colony stimulating factors and interleukin. Flt3L stimulates the proliferation and differentiation of various blood cell progenitor cells by activating FLT3
Alternative Fetal liver kinase 2, FLK-2
Accession P49772

Image & description

Fig.SDS-PAGE analysis of Mouse Flt-3 Ligand protein

Fig.SDS-PAGE analysis of Mouse Flt-3 Ligand protein

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