| Product name | Mouse CCL4 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse CCL4 (Ala24-Asn92) (P14097) was expressed with 6×His tag at the N-terminus |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measured by its ability to chemoattract human PBMCs using a concentration range of 20.0-200.0 ng/mL |
| Protein length | The recombinant Mouse CCL4 consists of 68 amino acids and predicts a molecular mass of 8.64 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | MIP-1b: Macrophage Inflammatory Protein-1β, ACT-2 |
| Formulation | Lyophilized from PBS, pH 7.4 |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 8.64 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | C-C Motif Chemokine Ligand 4 (CCL4) is a 7.66 kDa cytokine with 69 amino acid residues. CCL4, also named macrophage inflammatory protein-1β (MIP-1β), is mainly secreted from neutrophils, monocytes, B cells, T cells, fibroblasts, endothelial cells, and epithelial cells. In addition, CCL4 participates in immune responses, including recruitment of immune cells like lymphocytes, monocytes, and leukocytes, response to IL-1 and IFNγ, and production of TNF when CCL4 binds to CCR5. |
| Alternative | MIP-1b: Macrophage Inflammatory Protein-1β, ACT-2 |
| Accession | P14097 |
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