| Product name | Mouse BMP-4 protein , His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse BMP-4 (Lys303-Arg408)(P21275) with 6×His tag at the C-terminus was expressed |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measure by its ability to induce alkaline phosphatase production by ATDC5 cells.The ED₅₀ for this effect is <10 ng/mL.The specific activity of recombinant mouse BMP-4 is > 1 x 10⁵ IU/mg. |
| Protein length | The secreted Mouse BMP-4 consists of 106 amino acids and has a predicted molecular mass of 12.8 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | BMP-2B, DVR4 |
| Formulation | Lyophilized from sterile 20 mM sodium carbonate, pH 4.5 |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 12.88 kDa.The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse BMP-4 protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Bone morphogenetic protein 4 (BMP-4) is a member of transforming growth factor (TGF-β) family, which plays a vital role in human malignant tumors. BMP-4 treatment promoted the proliferation of HCC cells and induced autophagy. Mechanism study shows that autophagy induced by BMP-4 is mediated by activating JNK1/Bcl2 pathway. JNK1 inhibitor and JNK1 knockout can weaken the autophagy induced by BMP-4 and eliminate the growth of HCC cells promoted by BMP-4. BMP-4 promotes the proliferation of HCC through autophagy activation of JNK1/Bcl-2 signaling |
| Alternative | BMP-2B, DVR4 |
| Accession | P21275 |
Fig.SDS-PAGE analysis of Mouse BMP-4 protein
You must be logged in to post a review.
Reviews
There are no reviews yet.