| Product name | Mouse β-NGF protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse β-NGF protein (P01139)(Met121—Gly241)was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measure by its ability to induce TF-1 cells proliferation.The ED₅₀ for this effect is <1ng/mL.The specific activity of recombinant mouse beta-NGF is > 1 x 10⁶ IU/mg. |
| Protein length | The protein has a calculated MW of 14.41 kDa.The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis). |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | β-Nerve Growth Factor, NGF-β |
| Formulation | The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 14.41 kDa. The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse β-NGF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of Rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and Rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species |
| Alternative | β-Nerve Growth Factor, NGF-β |
| Accession | P01139 |
Fig.SDS-PAGE analysis of Mouse β-NGF protein
You must be logged in to post a review.
Reviews
There are no reviews yet.