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MDHC Polyclonal Antibody

MDHC Polyclonal Antibody

Views(3) Publications(0) Catalog no(ABP59246)
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Specification

Product name MDHC Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human MDHC protein
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:5000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Malate dehydrogenase, cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 36kD
Storage buffer PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MDH1 encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
Gene ID 4190
Alternative Malate dehydrogenase, cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase
Others The antibody detects endogenous levels of MDHC protein
Accession P40925
Observed Band(KD) 36

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