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MAGE-A5 Polyclonal Antibody

MAGE-A5 Polyclonal Antibody

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Specification

Product name MAGE-A5 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human MAGE-A5 at AA rangle: 40-120
Host Rabbit
Reactivity Human
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative MAGEA5; MAGE5; Melanoma-associated antigen 5; Cancer/testis antigen 1.5; CT1.5; MAGE-5 antigen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 36kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MAGEA5 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This MAGEA gene encodes a protein that is C-terminally truncated compared to other family members, and this gene can be alternatively interpreted to be a pseudogene. The protein is represented in this Gene record in accordance with the assumed protein-coding status defined in the literature. Read-through transcription exists between this gene and the upstream melanoma antigen family A, 10 (MAGEA10) gene.
Gene ID 4104
Alternative MAGEA5; MAGE5; Melanoma-associated antigen 5; Cancer/testis antigen 1.5; CT1.5; MAGE-5 antigen
Others MAGE-A5 Polyclonal Antibody detects endogenous levels of MAGE-A5 protein.
Accession P43359
Observed Band(KD) 36

Image & description

Fig. Western Blot analysis of 3T3 cells using MAGE-A5 Polyclonal Antibody.

Fig. Western Blot analysis of 3T3 cells using MAGE-A5 Polyclonal Antibody.

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