| Product name | Lamin B1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Lamin B1 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | LMNB1; LMN2; LMNB; Lamin-B1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 70kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | LMNB1 encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. |
| Gene ID | 4001 |
| Alternative | LMNB1; LMN2; LMNB; Lamin-B1 |
| Others | Lamin B1 Polyclonal Antibody detects endogenous levels of Lamin B1 protein. |
| Accession | P20700 |
| Observed Band(KD) | 70 |
Fig.1. Western Blot analysis of MCF7, SW480, PC-3, SKOV3, Mouse spleen, Mouse brain, Mouse liver, Mouse thymus cells using Lamin B1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.
Fig.6. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.
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