| Product name | IPKine™ Anti-GFP Magnetic IP Kit |
| Immunogen | Synthetic Peptide |
| Reactivity | Mammals, Bacteria |
| Applications | IP |
| Applications notes | Anti-Green Fluorescent Protein-Tag (GFP-Tag) Magnetic Beads are prepared by covalently coupling Anti-GFP-Tag Mouse Monoclonal Antibody to crosslinked Magnetic Beads. The Optimized Anti-GFP Magnetic Beads have more efficient antigen binding capacity. This kit provides two elution methods, including acid elution and denatured elution. |
| Conjugate | Magnetic Beads |
| Kit components | Non-Denaturing Lysis Buffer TBS (10×) Anti-GFP Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer SDS-PAGE Loading Buffer (5×) |
| Features & Benefits | 1.Efficient binding capacity: ≥0.6 mg GFP Tag protein/mL Magnetic Beads. • 2.Two elution methods: acid elution and denatured elution. • 3.The kit contains high-quality Anti-GFP magnetic beads and all validated immunoprecipitation necessary reagents. • 4.Ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules. |
| Storage instructions | Store according to the recommended storage conditions of each component, stable for 12 months. |
| Shipping | Gel pack with blue ice. |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
| Background | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kD) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish. The GFP has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. |
Figure. The immunoprecipitation effect of Anti-GFP Magnetic IP Kit used for GFP-Tag fusion protein. HEK293T cells were transfected with GFP-Tag plasmid. Lane 1 was whole cell lysate (WCL); Lane 2 was the immunoprecipitation sample of Mouse IgG Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer; Lane 3 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer. Lane 4 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by Elution Buffer. By using acid elution, only contained GFP-Tag fusion protein, did not contain heavy and light chains of antibody.
Author：Zhang, T., Shi, C., Hu, H. et al. Publication name：Nature communications IF：17.694
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