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Integrin β7 Polyclonal Antibody

Integrin β7 Polyclonal Antibody

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Specification

Product name Integrin β7 Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human Integrin β7 protein at amino acid sequence of 671-720
Host Rabbit
Reactivity Human,Mouse
Applications WB,ELISA,IHC
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Integrin beta-7; Gut homing receptor beta subunit

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 130kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ITGB7 encodes a protein that is a member of the integrin superfamily. Members of this family are adhesion receptors that function in signaling from the extracellular matrix to the cell. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The encoded protein forms dimers with an alpha4 chain or an alphaE chain and plays a role in leukocyte adhesion. Dimerization with alpha4 forms a homing receptor for migration of lymphocytes to the intestinal mucosa and Peyer's patches. Dimerization with alphaE permits binding to the ligand epithelial cadherin, a calcium-dependent adhesion molecule. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Gene ID 3695
Alternative Integrin beta-7; Gut homing receptor beta subunit
Others The antibody detects endogenous levels of Integrin β7
Accession P26010
Observed Band(KD) 130

Image & description

Fig.1. Western Blot analysis of Mouse-heart cells using primary antibody diluted at 1:1000 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.

Fig.1. Western Blot analysis of Mouse-heart cells using primary antibody diluted at 1:1000 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.

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