| Product name | IARS2 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human IARS2 at AA range: 40-120 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | IARS2; Isoleucine--tRNA ligase; mitochondrial; Isoleucyl-tRNA synthetase; IleRS |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 114kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. IARS2 encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. |
| Gene ID | 55699 |
| Alternative | IARS2; Isoleucine--tRNA ligase; mitochondrial; Isoleucyl-tRNA synthetase; IleRS |
| Others | IARS2 Polyclonal Antibody detects endogenous levels of IARS2 protein. |
| Accession | Q9NSE4 |
| Observed Band(KD) | 114 |
Fig.1. Western Blot analysis of various cells using IARS2 Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of NIH-3T3 cells using IARS2 Polyclonal Antibody diluted at 1:2000.
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