| Product name | Human VEGF165 protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human VEGF165 protein (Ala207-Arg395)(NP_001165097) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce HUVEC cells proliferation.The ED₅₀ for this effect is <5 ng/mL.The specific activity of recombinant human VEGF165 is approximately >1.4 x 10⁶ IU/mg. |
| Protein length | The Human VEGF165 protein consists of 189 amino acids and has a predicted molecular mass of 20.1 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen |
| Formulation | Lyophilized from sterile PBS, pH 8.0 |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 20.11 kDa. The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized Human VEGF165 protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | VEGF165, also known as Vascular Endothelial Growth Factor 165, is a protein that plays a critical role in angiogenesis, the process of forming new blood vessels from pre-existing ones. It belongs to the vascular endothelial growth factor family and is one of the most well-studied isoforms of VEGF. Human VEGF165 protein is a crucial regulator of angiogenesis, with implications in various physiological and pathological processes. Its study contributes to our understanding of blood vessel development, tissue repair, and the development of therapeutic strategies for diseases such as cancer |
| Alternative | VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen |
| Accession | NP_001165097 |
Fig.SDS-PAGE analysis of Human VEGF165 protein
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