| Product name | Human TGF-β3 protein |
| Sequence | MALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS with polyhistidine tag at the C-terminus. |
| Activity | Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant human TGF beta 3 is > 2 x 10⁷ IU/mg. |
| Protein length | The protein has a calculated MW of 13.66 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis). |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | ARVD, ARVD1, LDS5, RNHF, TGFB3, TGF-B3 |
| Formulation | The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 13.66 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile 10 mM HCl to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| Storage instructions | Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Transforming Growth Factors-Beta 3 (TGF-beta 3) is belonging to the TGF-β superfamily TGF-β3 is 86%, 91% similar to TGF-β1 and TGF-β2. TGF-beta 3 is a 12.8 kDa protein containing 412 amino acids, which could inhibitor of DNA synthesis, increases cellular proliferation, essential mediator of EMT in cardiac morphogenesis, and up-regulated by milk stasis and induces apoptosis in mammary gland epithelium during involution. |
| Alternative | ARVD, ARVD1, LDS5, RNHF, TGFB3, TGF-B3 |
| Accession | P10⁶00 |
Fig.SDS-PAGE analysis of Human TGF-β3 protein
Author：W Yan, M Shen, K Sun, S Li, J Miao, J Wang, J Xu Publication name：Biomedicines IF：4.7
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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